Active Ingredient: Ciprofloxacin
Isolates of A.
An allele of bla IMP was detected in one of these isolates, but the other 12 isolates either had carbapenemase-independent resistance, or undetectable carbapenemase activity combined with other resistance mechanisms.
In conclusion, carbapenems, colistin and minocycline retained greatest activity against the Acinetobacter isolates collected.
Tigecycline was less active than minocycline, but both agents overcame most tetracycline resistance. Introduction Acinetobacter spp.
Although carbapenemases are not always associated with high levels of phenotypic resistance in Acinetobacter spp.
Each isolate was tested with a panel of 14 established antimicrobial agents, including representatives of all the major antibiotic classes. There is a paucity of new drugs active against Acinetobacter—or any other Gram-negative pathogens—but tigecycline glycylcycline GAR-936 8 was included as a novel tetracycline known to evade both ribosomal- and efflux-mediated resistance to established analogues.
Also included were the established carbapenems and colistin, which have been used against infections caused by multiresistant Acinetobacter strains.
Species identification was confirmed centrally at Nottingham Public Health Laboratory. Data collected included the patient's age, sex, ward type, the site of isolation of organism and the susceptibility data generated by the source laboratory.
Identification Genomic groups were assigned by amplification of the tDNA spacer regions, 9 followed by separation of products on 1.
Susceptibility testing MICs for each isolate were determined on IsoSensitest agar with an inoculum of 104 to 105 cfu. Antimicrobial agents and ranges tested were: amikacin 0.
Susceptibilities were interpreted, where possible, using the breakpoints recommended for Acinetobacter spp. Testa, Wyeth-Ayerst, personal communication.
Results General A total of 595 of 649 isolates referred by the 54 sentinel laboratories were confirmed as Acinetobacter spp.
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