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Figure 2 Open in new tab Download slide Distribution of MICs of ciprofloxacin for Neisseria gonorrhoeae isolates from patients with treatment success white bars or failure black bars.
The antimicrobial susceptibilities of the pretreatment and posttreatment isolates from patients who had treatment failure were compared.
The MICs for pre- and posttreatment isolates were found to be similar. Penicillinase-producing N.
To determine the clonal relation among the isolates, and to confirm that treatment failure was due to resistance of the primary isolates to ciprofloxacin, rather than to reinfection, serogroup and serovar analyses were performed for all isolates.
All paired isolates were found to be of identical serogroup and serovar, as determined by means of a coagglutination method that used a panel of 14 monoclonal antibodies to protein I. All isolates, from both groups of patients, that expressed PIA belonged to the Arst serovar.
PCR—restriction fragment length polymorphism analysis of the isolates. To further establish that treatment failure was due to resistance of the primary isolates to ciprofloxacin, rather than to reinfection with resistant isolates of same serovar, the por gene was amplified from each pair of isolates by use of PCR and was cleaved with the Msp A 1 I restriction enzyme.
All paired isolates were found to be identical and to harbor either PIA or PIB, and they showed identical digestion patterns figure 3. Lanes 1, 5, and 9 denote pretreatment isolates 4, 15, 27, 52, and 59, respectively.
Lanes 2, 6, and 10 denote posttreatment isolates from the corresponding patients.
Lanes 1, 5, and 7 indicate pretreatment isolates 4, 15, 52, and 57, respectively. You should consult your health care professional before taking any drug, changing your diet, or commencing or discontinuing any course of treatment.
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